Swi/Snf chromatin remodeling regulates transcriptional interference and gene repression.
Mol Cell
; 84(16): 3080-3097.e9, 2024 Aug 22.
Article
em En
| MEDLINE
| ID: mdl-39043178
ABSTRACT
Alternative transcription start sites can affect transcript isoform diversity and translation levels. In a recently described form of gene regulation, coordinated transcriptional and translational interference results in transcript isoform-dependent changes in protein expression. Specifically, a long undecoded transcript isoform (LUTI) is transcribed from a gene-distal promoter, interfering with expression of the gene-proximal promoter. Although transcriptional and chromatin features associated with LUTI expression have been described, the mechanism underlying LUTI-based transcriptional interference is not well understood. Using an unbiased genetic approach followed by functional genomics, we uncovered that the Swi/Snf chromatin remodeling complex is required for co-transcriptional nucleosome remodeling that leads to LUTI-based repression. We identified genes with tandem promoters that rely on Swi/Snf function for transcriptional interference during protein folding stress, including LUTI-regulated genes. This study provides clear evidence for Swi/Snf playing a direct role in gene repression via a cis transcriptional interference mechanism.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Fatores de Transcrição
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Transcrição Gênica
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Proteínas Cromossômicas não Histona
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Nucleossomos
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Regiões Promotoras Genéticas
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Proteínas de Saccharomyces cerevisiae
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Montagem e Desmontagem da Cromatina
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article