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Optimization of the microbiological treatment of sperm for assisted reproductive technology (ART).
Bekon, Affoua-Melissa; Martiny, Delphine; Thomas, Anne-Laure; Miendje, Yvette; Debyttere, Anne-Laurence; Autin, Candice; Bertrand, Evelyne.
Afiliação
  • Bekon AM; Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), 322, Rue Haute, 1000 Brussels, Belgium; Centre PMA, Dpt Gynecology/Obstetric, IVF laboratory, UHC Saint-Pierre, 322, Rue Haute, 1000 Brussels, Belgium; Faculté de Médecine et
  • Martiny D; Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), 322, Rue Haute, 1000 Brussels, Belgium; Faculté de Médecine et Pharmacie,Université de Mons (UMONS), Mons, Belgium. Electronic address: delphine.martiny@lhub-ulb.be.
  • Thomas AL; Centre PMA, Dpt Gynecology/Obstetric, IVF laboratory, UHC Saint-Pierre, 322, Rue Haute, 1000 Brussels, Belgium.
  • Miendje Y; Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), 322, Rue Haute, 1000 Brussels, Belgium.
  • Debyttere AL; Department of Microbiology, Laboratoire Hospitalier Universitaire de Bruxelles-Universitair Laboratorium Brussel (LHUB-ULB), 322, Rue Haute, 1000 Brussels, Belgium.
  • Autin C; Centre PMA, Dpt Gynecology/Obstetric, IVF laboratory, UHC Saint-Pierre, 322, Rue Haute, 1000 Brussels, Belgium.
  • Bertrand E; Centre PMA, Dpt Gynecology/Obstetric, IVF laboratory, UHC Saint-Pierre, 322, Rue Haute, 1000 Brussels, Belgium. Electronic address: evelyne.bertrand@stpierre-bru.be.
J Microbiol Methods ; 224: 107004, 2024 Sep.
Article em En | MEDLINE | ID: mdl-39047945
ABSTRACT
In ART,1 optimized sperm preparation could separate spermatozoa from potentially harmful germs and cells, improving sperm parameters. This study compares the outcome of a routinely used two-layer density gradient with a three-layer protocol in terms of sperm and microbiological parameters. According to the results, a three-layer protocol was more effective.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Técnicas de Reprodução Assistida Limite: Humans / Male Idioma: En Ano de publicação: 2024 Tipo de documento: Article
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espermatozoides / Técnicas de Reprodução Assistida Limite: Humans / Male Idioma: En Ano de publicação: 2024 Tipo de documento: Article