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Generic semi-automated radiofluorination strategy for single domain antibodies: [18F]FB-labelled single domain antibodies for PET imaging of fibroblast activation protein-α or folate receptor-α overexpression in cancer.
Dierick, Herlinde; Navarro, Laurent; Ceuppens, Hannelore; Ertveldt, Thomas; Pombo Antunes, Ana Rita; Keyaerts, Marleen; Devoogdt, Nick; Breckpot, Karine; D'Huyvetter, Matthias; Lahoutte, Tony; Caveliers, Vicky; Bridoux, Jessica.
Afiliação
  • Dierick H; Molecular Imaging and Therapy Research Group (MITH), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103. Building K., 1090, Brussels, Belgium. herlinde.dierick@vub.be.
  • Navarro L; Nuclear Medicine Department, Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZ Brussel), Laarbeeklaan 101, 1090, Brussels, Belgium. herlinde.dierick@vub.be.
  • Ceuppens H; Precirix NV, Burgemeester Etienne Demunterlaan 3, 1090, Brussels, Belgium.
  • Ertveldt T; Laboratory for Molecular and Cellular Therapy (LCMT), Department of Biomedical Sciences, Translational Oncology Research Center, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103. Building E, 1090, Brussels, Belgium.
  • Pombo Antunes AR; Molecular Imaging and Therapy Research Group (MITH), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103. Building K., 1090, Brussels, Belgium.
  • Keyaerts M; Laboratory for Molecular and Cellular Therapy (LCMT), Department of Biomedical Sciences, Translational Oncology Research Center, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103. Building E, 1090, Brussels, Belgium.
  • Devoogdt N; Precirix NV, Burgemeester Etienne Demunterlaan 3, 1090, Brussels, Belgium.
  • Breckpot K; Molecular Imaging and Therapy Research Group (MITH), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103. Building K., 1090, Brussels, Belgium.
  • D'Huyvetter M; Molecular Imaging and Therapy Research Group (MITH), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103. Building K., 1090, Brussels, Belgium.
  • Lahoutte T; Laboratory for Molecular and Cellular Therapy (LCMT), Department of Biomedical Sciences, Translational Oncology Research Center, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103. Building E, 1090, Brussels, Belgium.
  • Caveliers V; Molecular Imaging and Therapy Research Group (MITH), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103. Building K., 1090, Brussels, Belgium.
  • Bridoux J; Precirix NV, Burgemeester Etienne Demunterlaan 3, 1090, Brussels, Belgium.
EJNMMI Radiopharm Chem ; 9(1): 54, 2024 Jul 24.
Article em En | MEDLINE | ID: mdl-39048805
ABSTRACT

BACKGROUND:

Radiofluorination of single domain antibodies (sdAbs) via N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB) has shown to be a promising strategy in the development of sdAb-based PET tracers. While automation of the prosthetic group (PG) [18F]SFB production, has been successfully reported, no practical method for large scale sdAb labelling has been reported. Therefore, we optimized and automated the PG production, enabling a subsequently efficient manual conjugation reaction to an anti-fibroblast activation protein (FAP)-α sdAb (4AH29) and an anti-folate receptor (FR)-α sdAb (2BD42). Both the alpha isoform of FAP and the FR are established tumour markers. FAP-α is known to be overexpressed mainly by cancer-associated fibroblasts in breast, ovarian, and other cancers, while its expression in normal tissues is low or undetectable. FR-α has an elevated expression in epithelial cancers, such as ovarian, brain and lung cancers. Non-invasive imaging techniques, such as PET-imaging, using tracers targeting specific tumour markers can provide molecular information over both the tumour and its environment, which aides in the diagnosis, therapy selection and assessment of the cancer treatment.

RESULTS:

[18F]SFB was synthesized using a fully automated three-step, one-pot reaction. The total procedure time was 54 min and results in [18F]SFB with a RCP > 90% and a RCY d.c. of 44 ± 4% (n = 13). The manual conjugation reaction after purification produced [18F]FB-sdAbs with a RCP > 95%, an end of synthesis activity > 600 MBq and an apparent molar activity > 10 GBq/µmol. Overall RCY d.c., corrected to the trapping of [18F]F- on the QMA, were 9% (n = 1) and 5 ± 2% (n = 3) for [18F]FB-2BD42 and [18F]FB-4AH29, respectively.

CONCLUSION:

[18F]SFB synthesis was successfully automated and upscaled on a Trasis AllInOne module. The anti-hFAP-α and anti-hFR-α sdAbs were radiofluorinated, yielding similar RCYs d.c. and RCPs, showing the potential of this method as a generic radiofluorination strategy for sdAbs. The radiofluorinated sdAbs showed a favourable biodistribution pattern and are attractive for further characterization as new PET tracers for FAP-α and FR-α imaging.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Ano de publicação: 2024 Tipo de documento: Article