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Chlamydia suis undergoes interclade recombination promoting Tet-island exchange.
Seth-Smith, Helena; Bommana, Sankhya; Dean, Deborah; Read, Timothy D; Marti, Hanna.
Afiliação
  • Seth-Smith H; Institute of Veterinary Pathology, University of Zurich, Zurich, Switzerland.
  • Bommana S; Division of Infectious Diseases and Global Health, Departments of Medicine and Pediatrics, University of California San Francisco School of Medicine, San Francisco, CA, USA.
  • Dean D; Division of Infectious Diseases and Global Health, Departments of Medicine and Pediatrics, University of California San Francisco School of Medicine, San Francisco, CA, USA.
  • Read TD; Joint Graduate Program in Bioengineering, University of California Berkeley, University of California San Francisco, Berkeley and San Francisco, CA, USA.
  • Marti H; Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA.
BMC Genomics ; 25(1): 724, 2024 Jul 26.
Article em En | MEDLINE | ID: mdl-39060998
ABSTRACT

BACKGROUND:

The obligate intracellular bacterial family Chlamydiaceae comprises a number of different species that cause disease in various vertebrate hosts including humans. Chlamydia suis, primarily found in the gastrointestinal tract of pigs, is the only species of the Chlamydiaceae family to have naturally gained tetracycline resistance (TetR), through a genomic island (Tet-island), integrated into the middle of chromosomal invasin-like gene inv. Previous studies have hypothesised that the uptake of the Tet-island from a host outside the Chlamydiaceae family was a unique event, followed by spread among C. suis through homologous recombination. In vitro recombination studies have shown that Tet-island exchange between C. suis strains is possible. Our aim in this study was to gain a deeper understanding of the interclade recombination of the Tet-island, among currently circulating C. suis field strains compared to in vitro-generated recombinants, using published whole genome sequences of C. suis field strains (n = 35) and in vitro-generated recombinants (n = 63).

RESULTS:

We found that the phylogeny of inv better reflected the phylogeny of the Tet-island than that of the whole genome, supporting recombination rather than site-specific insertion as the means of transfer. There were considerable differences between the distribution of recombinations within in vitro-generated strains compared to that within the field strains. These differences are likely because in vitro-generated recombinants were selected for a tetracycline and rifamycin/rifampicin resistant background, leading to the largest peak of recombination across the Tet-island. Finally, we found that interclade recombinations across the Tet-island were more variable in length downstream of the Tet-island than upstream.

CONCLUSIONS:

Our study supports the hypothesis that the occurrence of TetR strains in both clades of C. suis came about through interclade recombination after a single ancestral horizontal gene transfer event.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Recombinação Genética / Resistência a Tetraciclina / Chlamydia / Ilhas Genômicas Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Recombinação Genética / Resistência a Tetraciclina / Chlamydia / Ilhas Genômicas Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article