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A novel AP2/ERF transcription factor, NtERF10, positively regulates plant height in tobacco.
Xu, Li; Xu, Yong; Jiang, Jia-Rui; Cheng, Chang-Xin; Yang, Wen-Wu; Deng, Le-le; Mi, Qi-Li; Zeng, Wan-Li; Li, Jing; Gao, Qian; Xiang, Hai-Ying; Li, Xue-Mei.
Afiliação
  • Xu L; China Tobacco Yunnan Industrial Co., Ltd., Kunming, China.
  • Xu Y; China Tobacco Yunnan Industrial Co., Ltd., Kunming, China.
  • Jiang JR; China Tobacco Yunnan Industrial Co., Ltd., Kunming, China.
  • Cheng CX; Hongyun honghe Tobacco Group Co. Ltd, Kunming, China.
  • Yang WW; China Tobacco Yunnan Industrial Co., Ltd., Kunming, China.
  • Deng LL; China Tobacco Yunnan Industrial Co., Ltd., Kunming, China.
  • Mi QL; China Tobacco Yunnan Industrial Co., Ltd., Kunming, China.
  • Zeng WL; China Tobacco Yunnan Industrial Co., Ltd., Kunming, China.
  • Li J; China Tobacco Yunnan Industrial Co., Ltd., Kunming, China.
  • Gao Q; China Tobacco Yunnan Industrial Co., Ltd., Kunming, China.
  • Xiang HY; China Tobacco Yunnan Industrial Co., Ltd., Kunming, China.
  • Li XM; China Tobacco Yunnan Industrial Co., Ltd., Kunming, China. xmlikm@126.com.
Transgenic Res ; 33(4): 195-210, 2024 Aug.
Article em En | MEDLINE | ID: mdl-39105946
ABSTRACT
Ethylene response factors have been shown to be involved in the effects of plant developmental processes and to regulate stress tolerance. The aim of this study was to recognize the regulatory mechanisms of ethylene response factors on tobacco plant height. In this study, a gene-edited mutant (ERF10-KO) and wild type (WT) were utilized as experimental materials. Transcriptome and metabolome analyses were used to investigate the regulatory mechanism of NtERF10 gene editing on plant height in tobacco. Here, through the analysis of differentially expressed genes (DEGs), 2051 genes were upregulated and 1965 genes were downregulated. We characterized the different ERF10-KO and WT plant heights and identified key genes for photosynthesis, the plant hormone signal transduction pathway and the terpene biosynthesis pathway. NtERF10 was found to affect the growth and development of tobacco by regulating the expression levels of the PSAA, PSBA, GLY17 and GGP3 genes. Amino acid metabolism was analyzed by combining analyses of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs). In addition, we found that members of the bHLH, NAC, MYB, and WRKY transcription factor families have vital roles in regulating plant height. This study not only provides important insights into the positive regulation of the ethylene response factor NtERF10 on plant height during plant growth and development but also provides new research ideas for tobacco molecular breeding.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Nicotiana / Fatores de Transcrição / Regulação da Expressão Gênica de Plantas Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Nicotiana / Fatores de Transcrição / Regulação da Expressão Gênica de Plantas Idioma: En Ano de publicação: 2024 Tipo de documento: Article