The FXR1 network acts as a signaling scaffold for actomyosin remodeling.
Cell
; 187(18): 5048-5063.e25, 2024 Sep 05.
Article
em En
| MEDLINE
| ID: mdl-39106863
ABSTRACT
It is currently not known whether mRNAs fulfill structural roles in the cytoplasm. Here, we report the fragile X-related protein 1 (FXR1) network, an mRNA-protein (mRNP) network present throughout the cytoplasm, formed by FXR1-mediated packaging of exceptionally long mRNAs. These mRNAs serve as an underlying condensate scaffold and concentrate FXR1 molecules. The FXR1 network contains multiple protein binding sites and functions as a signaling scaffold for interacting proteins. We show that it is necessary for RhoA signaling-induced actomyosin reorganization to provide spatial proximity between kinases and their substrates. Point mutations in FXR1, found in its homolog FMR1, where they cause fragile X syndrome, disrupt the network. FXR1 network disruption prevents actomyosin remodeling-an essential and ubiquitous process for the regulation of cell shape, migration, and synaptic function. Our findings uncover a structural role for cytoplasmic mRNA and show how the FXR1 RNA-binding protein as part of the FXR1 network acts as an organizer of signaling reactions.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Actomiosina
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RNA Mensageiro
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Transdução de Sinais
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Proteínas de Ligação a RNA
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Proteína rhoA de Ligação ao GTP
Limite:
Humans
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article