MADDD-seq, a novel massively parallel sequencing tool for simultaneous detection of DNA damage and mutations.
Nucleic Acids Res
; 52(16): e76, 2024 Sep 09.
Article
em En
| MEDLINE
| ID: mdl-39149908
ABSTRACT
Our genome is exposed to a wide variety of DNA-damaging agents. If left unrepaired, this damage can be converted into mutations that promote carcinogenesis or the development of genetically inherited diseases. As a result, researchers and clinicians require tools that can detect DNA damage and mutations with exceptional sensitivity. In this study, we describe a massively parallel sequencing tool termed Mutation And DNA Damage Detection-seq (MADDD-seq) that is capable of detecting O6-methyl guanine lesions and mutations simultaneously, with a single assay. To illustrate the dual capabilities of MADDD-seq, we treated WT and DNA repair deficient yeast cells with the DNA-damaging agent MNNG and tracked DNA lesions and mutations over a 24-h time period. This approach allowed us to identify thousands of DNA adducts and mutations in a single sequencing run and gain deep insight into the kinetics of DNA repair and mutagenesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Dano ao DNA
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Reparo do DNA
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Sequenciamento de Nucleotídeos em Larga Escala
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Mutação
Idioma:
En
Ano de publicação:
2024
Tipo de documento:
Article