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Matrix stiffness regulates the triad communication of adipocytes/macrophages/endothelial cells through CXCL13.
Choisez, Arthur; Ishihara, Seiichiro; Ishii, Takuro; Xu, Yidan; Firouzjah, Sepideh D; Haga, Hisashi; Nagatomi, Ryoichi; Kusuyama, Joji.
Afiliação
  • Choisez A; Department of Biosignals and Inheritance, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan; Division of Biomedical Engineering for Health and Welfare, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan.
  • Ishihara S; Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan.
  • Ishii T; Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan.
  • Xu Y; Department of Medicine and Science in Sports and Exercise, Tohoku University School of Medicine, Sendai, Japan.
  • Firouzjah SD; Department of Medicine and Science in Sports and Exercise, Tohoku University School of Medicine, Sendai, Japan.
  • Haga H; Department of Advanced Transdisciplinary Sciences, Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan.
  • Nagatomi R; Department of Biosignals and Inheritance, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan; Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Japan.
  • Kusuyama J; Department of Biosignals and Inheritance, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan; Division of Biomedical Engineering for Health and Welfare, Graduate School of Biomedical Engineering, Tohoku University, Sendai, Japan; Frontier Researc
J Lipid Res ; 65(9): 100620, 2024 Sep.
Article em En | MEDLINE | ID: mdl-39151591
ABSTRACT
Adipose tissue remodeling and plasticity are dynamically regulated by the coordinated functions of adipocytes, macrophages, and endothelial cells and extracellular matrix (ECM) that provides stiffness networks in adipose tissue component cells. Inflammation and fibrosis are crucial exogenous factors that dysregulate adipose tissue functions and drastically change the mechanical properties of the ECM. Therefore, communication among the ECM and adipose tissue component cells is necessary to understand the multifaceted functions of adipose tissues. To obtain in vivo stiffness, we used genipin as a crosslinker for collagen gels. Meanwhile, we isolated primary adipocytes, macrophages, and endothelial cells from C57BL/6J mice and incubated these cells in the differentiation media on temperature-responsive culture dishes. After the differentiation, these cell sheets were transferred onto genipin-crosslinked collagen gels with varying matrix stiffness. We found that inflammatory gene expressions were induced by hard matrix, whereas antiinflammatory gene expressions were promoted by soft matrix in all three types of cells. Interestingly, the coculture experiments of adipocytes, macrophages, and endothelial cells showed that the effects of soft or hard matrix stiffness stimulation on adipocytes were transmitted to the distant adipose tissue component cells, altering their gene expression profiles under normal matrix conditions. Finally, we identified that a hard matrix induces the secretion of CXCL13 from adipocytes, and CXCL13 is one of the important transmitters for stiffness communication with macrophages and endothelial cells. These findings provide insight into the mechanotransmission into distant cells and the application of stiffness control for chronic inflammation in adipose tissues with metabolic dysregulation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adipócitos / Células Endoteliais / Matriz Extracelular / Quimiocina CXCL13 / Macrófagos / Camundongos Endogâmicos C57BL Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adipócitos / Células Endoteliais / Matriz Extracelular / Quimiocina CXCL13 / Macrófagos / Camundongos Endogâmicos C57BL Limite: Animals Idioma: En Ano de publicação: 2024 Tipo de documento: Article