Development of RPA-Cas12a assay for rapid and sensitive detection of Pneumocystis jirovecii.

Liu, Qiming; Zeng, Hao; Wang, Ting; Ni, HongXia; Li, Yongdong; Qian, Weidong; Fang, Ting; Xu, Guozhang

Liu, Qiming; Zeng, Hao; Wang, Ting; Ni, HongXia; Li, Yongdong; Qian, Weidong; Fang, Ting; Xu, Guozhang.

Afiliação

Liu Q; School of Biological and Pharmaceutical Sciences, Shaanxi University of Science and Technology, Xi'an, 710021, China.
Zeng H; School of Biological and Pharmaceutical Sciences, Shaanxi University of Science and Technology, Xi'an, 710021, China.
Wang T; School of Biological and Pharmaceutical Sciences, Shaanxi University of Science and Technology, Xi'an, 710021, China.
Ni H; Ningbo Municipal Key Laboratory of Virology, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, China.
Li Y; Ningbo Municipal Key Laboratory of Virology, Ningbo Municipal Center for Disease Control and Prevention, Ningbo, 315010, China.
Qian W; School of Biological and Pharmaceutical Sciences, Shaanxi University of Science and Technology, Xi'an, 710021, China. qianwd@sust.edu.cn.
Fang T; Department of Epidemiology and Biostatistics, School of Public Health Health Science Center of Ningbo University, Ningbo, 315211, China. fangting@nbu.edu.cn.
Xu G; Department of Epidemiology and Biostatistics, School of Public Health Health Science Center of Ningbo University, Ningbo, 315211, China. xuguozhang@nbu.edu.cn.

BMC Microbiol ; 24(1): 314, 2024 Aug 26.

Article em En | MEDLINE | ID: mdl-39187803

Assuntos

Técnicas de Amplificação de Ácido Nucleico; Pneumocystis carinii; Sensibilidade e Especificidade; Pneumocystis carinii/genética; Pneumocystis carinii/isolamento & purificação; Técnicas de Amplificação de Ácido Nucleico/métodos; Humanos; Pneumonia por Pneumocystis/diagnóstico; Pneumonia por Pneumocystis/microbiologia; Técnicas de Diagnóstico Molecular/métodos; Endodesoxirribonucleases/genética; Endodesoxirribonucleases/metabolismo; Proteínas Associadas a CRISPR/genética; DNA Fúngico/genética; Recombinases/metabolismo; Recombinases/genética; Proteínas de Bactérias

Palavras-chave

Pneumocystis jirovecii; CRISPR-Cas12a; Detection; Recombinase polymerase amplification; Resource-limited settings

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sensibilidade e Especificidade / Técnicas de Amplificação de Ácido Nucleico / Pneumocystis carinii Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

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