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Anticancer Activity and Mechanism of Action of Couroupita guianensis Bark Decoction in Gastric Adenocarcinoma Cancer Cell Line.
Pisanti, Simona; Penna, Serena; Sposito, Silvia; Esposito, Tiziana; Mencherini, Teresa; Celano, Rita; Re, Tania; Aquino, Rita Patrizia; Martinelli, Rosanna.
Afiliação
  • Pisanti S; Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, 84081 Baronissi, SA, Italy.
  • Penna S; Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, 84081 Baronissi, SA, Italy.
  • Sposito S; Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, 84081 Baronissi, SA, Italy.
  • Esposito T; Department of Pharmacy, University of Salerno, 84084 Fisciano, SA, Italy.
  • Mencherini T; UNESCO Chair Salerno Plantae Medicinales Mediterraneae, University of Salerno, 84084 Fisciano, SA, Italy.
  • Celano R; Department of Pharmacy, University of Salerno, 84084 Fisciano, SA, Italy.
  • Re T; UNESCO Chair Salerno Plantae Medicinales Mediterraneae, University of Salerno, 84084 Fisciano, SA, Italy.
  • Aquino RP; Department of Pharmacy, University of Salerno, 84084 Fisciano, SA, Italy.
  • Martinelli R; UNESCO Chair Salerno Plantae Medicinales Mediterraneae, University of Salerno, 84084 Fisciano, SA, Italy.
Int J Mol Sci ; 25(17)2024 Aug 24.
Article em En | MEDLINE | ID: mdl-39273132
ABSTRACT
Couroupita guianensis, a medicinal plant autochthonal to South America and South India, is widely used in the ethnomedicine of the indigenous peoples of these regions thanks to its alleged antimicrobial, anti-inflammatory, antioxidant and wound-healing properties. The majority of studies have mainly analyzed organic extracts of the Indian plant's flowers and leaves, with limited research on its bark decoction, traditionally used in Amazonian shamanic medicine. In this study, we investigated the anticancer effects of the bark decoction and its main fractions obtained through chromatographic separation, as well as the underlying molecular mechanisms in AGS gastric cancer cells. Viability, cell proliferation, cell cycle, apoptosis and protein expression related to these processes were evaluated. Both the bark decoction and fraction III significantly inhibited cell viability, and the cytotoxic effect was linked to cell cycle blockade and the induction of apoptosis also through an engulfment of the autophagic flux. Increased expression or activation of the key proteins (p53, p21, cdk2, Bak, caspases, pAMPK, pAkt, beclin, p62 and LC3BII) involved in these processes was observed. The results obtained confirmed an important anticancer effect of C. guianensis bark decoction, providing scientific validation for its use in traditional medicine and highlighting its potential as a therapeutic agent against gastric cancer.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Extratos Vegetais / Apoptose / Casca de Planta / Proliferação de Células Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Extratos Vegetais / Apoptose / Casca de Planta / Proliferação de Células Limite: Humans Idioma: En Ano de publicação: 2024 Tipo de documento: Article