The catalysis by heparin of the reaction between thrombin and antithrombin.
Biochim Biophys Acta
; 870(1): 92-101, 1986 Mar 07.
Article
em En
| MEDLINE
| ID: mdl-3947650
ABSTRACT
Fluorescence polarization has been used to study the kinetics of the combination of thrombin with antithrombin and its catalysis by the polysaccharide heparin. The heparin-catalysed combination of thrombin and antithrombin is saturable with respect to both thrombin and antithrombin. The rate-determining step of the reaction is approximately 1.7 s-1. The kinetics observed can be explained by proposing that the catalyst of the reaction is not heparin alone but a complex of heparin and antithrombin (bound at the high-affinity site). The temperature dependence of the heparin-catalysed reaction is indistinguishable from that of the uncatalysed reaction. This coincidence is consistent with the rate-limiting step being the same in both cases.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Heparina
/
Trombina
/
Antitrombinas
/
Polarização de Fluorescência
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article