Intracranial application of substances in the unrestrained, awake rat by pressure injection through glass micropipettes.
J Neurosci Methods
; 13(1): 1-8, 1985 Mar.
Article
em En
| MEDLINE
| ID: mdl-3990341
A method is described which allows intracranial injection of drug solutions through glass micropipettes (tip diameter 15 micron) in the freely moving rat by use of air pressure. Compared with conventional injection methods through steel cannulae this method has the advantages of (1) minimal destruction of brain tissue, (2) precise injection of small volumes of solutions (1 nl to several microliters), (3) simultaneous recording of local EEG activity, and (4) usage of multipipette assemblies.
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MEDLINE
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Encéfalo
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Mapeamento Encefálico
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Injeções
Limite:
Animals
Idioma:
En
Ano de publicação:
1985
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Article