Defects in DNA and globin messenger RNA in homozygotes for hemoglobin Lepore.
J Clin Invest
; 63(4): 736-42, 1979 Apr.
Article
em En
| MEDLINE
| ID: mdl-438334
ABSTRACT
Globin messenger RNA (mRNA) isolated from three patients homozygous for hemoglobin Lepore is shown to have a marked reduction of the amount of beta-like globin mRNA (Lepore-globin mRNA sequences) compared with alpha-globin mRNA by molecular hybridization. The relative amounts of alpha- and Lepore mRNA are similar to the amounts of alpha- and Lepore globin synthesized in intact cells and by isolated mRNA in a cell-free system. It is also demonstrated that Lepore-globin mRNA can completely hybridize to full-length or nearly full-length beta-globin specific complementary DNA and protect it from nuclease digestion, indicating close homology between the delta-mRNA sequences present in Lepore mRNA and the beta-complementary-DNA probe. We have also quantitated the numbers of beta-like globin gene sequences in genomic Lepore DNA by molecular hybridization and demonstrated a reduction in their number consistent with the Lepore gene being a delta beta-gene fusion product.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Globinas
/
Hemoglobinas Anormais
/
RNA Mensageiro
Limite:
Adolescent
/
Child, preschool
/
Humans
/
Male
Idioma:
En
Ano de publicação:
1979
Tipo de documento:
Article