Murine typhus toxin: studies on identification of the neutralizing factor present in normal human serum.
Infect Immun
; 6(3): 232-9, 1972 Sep.
Article
em En
| MEDLINE
| ID: mdl-4629256
ABSTRACT
Studies were made on the isolation and identification of the Rickettsia typhi toxin-neutralizing factor (TNF) previously demonstrated in normal human serum. By means of various methods of separating serum proteins, such as filtration on Sephadex G-200, dextran precipitation, hydroxyapatite chromatography, and ultracentrifugation, TNF was found to be closely associated with purified serum beta-lipoprotein, although no serological relationship with this protein was demonstrated. Lipase as well as trypsin digestion of purified preparations of beta-lipoprotein destroyed the TN activity. No evidence was obtained for an association of TNF with the immunoglobulins or with any serum protein other than beta-lipoprotein. Further studies revealed that (i) serum specimens with TN titers of 11024 and others with titers of 18 or less contained the same concentration of beta-lipoprotein; (ii) purified preparations of beta-lipoprotein isolated from TNF positive and negative sera, and which had the same protein concentration, differed as much as 250-fold in TN titer; and (iii) the TN activity of a serum could be removed by absorption with antiserum to beta-lipoprotein from a positive donor, but not with antiserum to beta-lipoprotein from a negative donor.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tifo Endêmico Transmitido por Pulgas
/
Antitoxinas
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1972
Tipo de documento:
Article