Separation of type 2 toxins of Vibrio cholerae.
Science
; 165(3895): 808-9, 1969 Aug 22.
Article
em En
| MEDLINE
| ID: mdl-5804723
ABSTRACT
Choleragenic toxin was separated from vascular permeability factor by ion-exchange chromatography of supernatants of dialyzed peptone cultures of Vibrio cholerae. The choleragenic toxin eluted from columns of QAE-Sephadex with low-ionicity systems is free of permeability factor activity. Further elution of these columns with 0.5M NaCl removes both the permeability factor and residual choleragenic toxin. When this latter material is chromatographed on columns of carboxymethyl-Sephadex, the permeability factor toxin is eluted by 0.02M phosphate buffer and is free of choleragenic activity. Therefore, choleragenic and permeability factor activities of the type 2 cholera toxins are different and can be separated by these procedures.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Toxinas Biológicas
/
Vibrio
/
Vibrioses
Limite:
Animals
Idioma:
En
Ano de publicação:
1969
Tipo de documento:
Article