Immunoglobulin genes of the kappa light chain type from two human lymphoid cell lines are closely related.
Nucleic Acids Res
; 12(18): 6995-7006, 1984 Sep 25.
Article
em En
| MEDLINE
| ID: mdl-6091049
As a first step in our studies of functionally rearranged K genes of man we cloned the germline JK-CK region from placenta DNA employing a mouse JK clone as hybridization probe. Subclones of the human JK-CK region were then used to characterize and clone the rearranged K genes of the lymphoid cell lines Walker and Daudi. The Walker cell line contains one rearranged and one germline K allele (K+,KO; ref. 1). Only one K gene was found in Daudi cells (K+). Restriction mapping and DNA sequencing showed, that the rearranged K genes from both cell lines are closely related. These features make the two cell lines particularly suitable for studies on the chromatin structure of K light chain genes. The 5' flanks of the two genes (388 bp) are identical while there is a 12% divergence between the VK gene segments themselves. This situation may reflect somatic mutation processes and/or gene conversion like events.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cadeias kappa de Imunoglobulina
/
Cadeias Leves de Imunoglobulina
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Genes
Limite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article