Expression of liver phenotypes in cultured mouse hepatoma cells.
J Natl Cancer Inst
; 64(4): 809-19, 1980 Apr.
Article
em En
| MEDLINE
| ID: mdl-6102619
ABSTRACT
Mouse hepatoma cells were established in vitro as a permanently growing line designated Hepa. The mass population and a subclone were characterized for their karyotype and their retention of liver-specific properties. An examination of 17 hepatic traits revealed that the cell lines secreted several serum proteins. The activities of a number of liver-specific enzymes, however, appeared to be absent in these cells. The identification of differentiated properties of cultured hepatoma cells permits the use of these lines in a variety of studies such as cell hybridization, biochemical analysis of tissue-specific gene products, and the modulation of expression of genes governing differentiated phenotypes. This report presents the analysis of a broad spectrum of characteristics and thereby describes one of the most fully defined hepatoma cell lines of murine origin in the literature.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Hepáticas Experimentais
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1980
Tipo de documento:
Article