HBsAg-like structures in immunosuppressed mice inoculated with human hepatitis B virus.
Virchows Arch B Cell Pathol Incl Mol Pathol
; 40(2): 249-61, 1982 Aug.
Article
em En
| MEDLINE
| ID: mdl-6127839
ABSTRACT
Thymectomised and irradiated DBA/2 mice were injected intraperitoneally with human serum containing high titer of HBsAg, and were positive for HBsAg. Through the entire experiment neither degenerative and inflammatory lesions nor hepatitis B virus antigens could be detected in the liver of these animals by histomorphology and immunofluorescence, respectively. The sera of all these mice were negative for HBsAg by radioimmunoassay. By electron microscopy, however, increasing amounts of filaments and round particles measuring 20-22 nm in diameter could be observed in the endoplasmic reticulum of the mouse hepatocytes from the 8th day following injection. From the 90th day after inoculation the number of the filaments increased in an extreme degree. After fixation with KMnO4 and EDTA preferential staining, the filaments proved to be highly electrondense. According to the authors the filaments observed in mouse livers are lipoproteins produced by the hepatocytes in response to HBV inoculation. The appearance of the filaments is HBsAg-like, though their immunological characteristics become modified.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vacinas Virais
/
Vírus da Hepatite B
/
Antígenos de Superfície da Hepatite B
/
Fígado
Limite:
Animals
Idioma:
En
Ano de publicação:
1982
Tipo de documento:
Article