The use of the peroxidase reaction to obliterate staining of eosinophils by fluorescein-labelled conjugates.

ABSTRACT

The staining of eosinophils by fluorescein conjugates can be obliterated by utilising the peroxidase activity of the eosinophil. The brown reaction product of 3,4,3',4'-tetraaminobiphenyl hydrochloride (DAB) with hydrogen peroxide was more effective than Lendrum's stain. A satisfactory alternative to the possibly carcinogenic DAB was 2,7-fluorenediamine.