[Escherichia coli K-12 mutants with an increased efficiency of plasmid transformation]. / Izuchenie mutantov Escherichia coli K-12 s povyshennoi éffektivnost'iu plazmidnoi transformatsii.
Genetika
; 19(3): 375-80, 1983 Mar.
Article
em Ru
| MEDLINE
| ID: mdl-6221967
ABSTRACT
Escherichia coli K-12 mutants with an enhanced efficiency of plasmid transformation were obtained. In all the mutants, the efficiency of transfection with lambda vir phage DNA was changed, in comparison to the parent strain. However, these changes did not always correlate strictly with plasmid transformation alterations. For instance, two mutants with an increased plasmid transformation efficiency demonstrated 50-fold decrease in the level of transfection with lambda phage DNA. Polyacrylamide gel electrophoresis points to both quantitative and qualitative differences in protein composition of the mutant cell envelopes, as compared with the parent strain.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Transformação Bacteriana
/
Escherichia coli
/
Mutação
Tipo de estudo:
Qualitative_research
Idioma:
Ru
Ano de publicação:
1983
Tipo de documento:
Article