Clonal growth of primary cultures of rabbit ear chondrocytes in a lipid-supplemented defined medium.
Exp Cell Res
; 145(2): 415-23, 1983 May.
Article
em En
| MEDLINE
| ID: mdl-6222911
ABSTRACT
Clonal growth of primary cultures of rabbit ear chondrocytes in a defined medium without serum or other undefined additives has been achieved. The clonal inoculum is a suspension of fully differentiated chondrocytes prepared by collagenase digestion of rabbit ear cartilage and used with no prior adaptation or selection in culture. When inoculated into medium MCDB 104 supplemented with 100 ng/ml fibroblast growth factor (FGF), 1 microgram/ml insulin, and 5 micrograms/ml of a lipid supplement previously developed for human fibroblasts, the isolated chondrocytes undergo clonal multiplication to form large colonies of epithelial-like cells. Colonies grown in the defined medium for 14 days accumulate at their centers refractile cartilage-like matrix that is stained by acidified Alcian green, although the amount is significantly less than with undefined additives. This system opens the way for detailed studies, in a defined background medium, of factors that regulate phenotypic expression of cartilage-like differentiated properties.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cartilagem
/
Lipídeos
Limite:
Animals
Idioma:
En
Ano de publicação:
1983
Tipo de documento:
Article