High-level expression of human interferon gamma in Escherichia coli under control of the pL promoter of bacteriophage lambda.
Gene
; 28(1): 55-64, 1984 Apr.
Article
em En
| MEDLINE
| ID: mdl-6234201
ABSTRACT
Several recombinant plasmids have been constructed which direct high-level synthesis of mature human interferon gamma (IFN-gamma) in Escherichia coli using the inducible leftward promoter pL of phage lambda followed by a translational initiator region derived either from the phage MS2 replicase gene or the E. coli tryptophan attenuator region. Under these conditions, IFN levels of up to 25% of the total cellular protein can be achieved. The highest levels were obtained when a terminator of transcription was cloned downstream from the IFN-gamma sequence. IFN-gamma was almost entirely found in the initial pellet fraction and not in soluble extracts. Co-induction of the lysis genes derived from phage MS2 or from phage lambda, inserted downstream from the IFN-gamma sequence, did not enhance the biological activity present in the supernatant fraction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interferon gama
/
Bacteriófago lambda
/
Escherichia coli
Limite:
Humans
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article