Nucleotide sequences of two regions of the human genome containing tRNAAsn genes.
Gene
; 28(2): 257-62, 1984 May.
Article
em En
| MEDLINE
| ID: mdl-6234206
ABSTRACT
The primary structures of two human tRNAAsn genes and 600-700 nucleotides of their flanking regions have been determined from two separate isolates of a fetal DNA library in phage lambda vector. The tRNA gene from one clone differs from the major mammalian tRNAAsn by a single base substitution at position 47, with an A replacing a G, while the tRNAAsn gene from the second clone has base substitutions at positions 17 and 65, with a G replacing a C and a T replacing a C, respectively. The sequences of the noncoding 5'- and 3'-flanking regions of both clones are over 90% homologous. As with other mammalian tRNA genes, these two human tRNAAsn genes contain CTTTTPu, which might act as a transcription termination signal, 11 bp 3' to the structural gene. In vitro transcription experiments in a HeLa cell extract demonstrate that both cloned tRNAAsn genes can be transcribed and processed to mature-sized tRNAs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aspartato-tRNA Ligase
/
Transcrição Gênica
/
Aminoacil-RNA de Transferência
Limite:
Humans
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article