Electron spin resonance studies of changes in membrane fluidity of Chinese hamster ovary cells during the cell cycle.
Biochim Biophys Acta
; 602(1): 117-26, 1980 Oct 16.
Article
em En
| MEDLINE
| ID: mdl-6251882
ABSTRACT
Electron spin resonance (ESR) spin-label methods were used with 5-doxyl-stearic acid as a probe to investigate membrane fluidity of Chinese hamster ovary (CHO) cells during the cell cycle. A 35 GHz ESR technique was developed to study membrane fluidity of intact cells. This technique requires only about 1/6 the amount of cells compared to the conventional spin-label techniques. With this technique we observed a cyclic change of membrane fluidity during the cell cycle of CHO cells cells in mitosis had the highest membrane fluidity, whereas cells in G1 and early S phases had the lowest membrane fluidity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ovário
/
Fluidez de Membrana
Limite:
Animals
Idioma:
En
Ano de publicação:
1980
Tipo de documento:
Article