Decrease in Na+,K+-ATPase activity and [3H]ouabain binding sites in sarcolemma prepared from hearts of spontaneously hypertensive rats.
Hypertension
; 5(5): 682-8, 1983.
Article
em En
| MEDLINE
| ID: mdl-6311739
ABSTRACT
Na+,K+-ATPase activity, phosphorylation, and [3H]ouabain binding in sarcolemma isolated from spontaneously hypertensive rat (SHR) hearts were compared to the same parameters in sarcolemma from normotensive rat (WKY) hearts. Sarcolemma prepared from SHR heart contained significantly less ouabain-inhibitable ATPase activity than sarcolemma from WKY heart. No significant differences in sarcolemmal protein content or recovery were noted between the two groups. The numbers of phosphorylation sites and ouabain binding sites were lower for SHR hearts than for WKY hearts. The KD values for ouabain binding were the same (0.30 muM) in cardiac sarcolemma of SHR and WKY. The I50 values for inhibition by ouabain of Na+,K+-ATPase were also the same for both groups (SHR = 49 microM; WKY = 44 microM). These data suggest that the decrease of cardiac sarcolemmal Na+,K+-ATPase activity in SHR hearts is due to a decrease in the number of active sites.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ouabaína
/
Ratos Endogâmicos
/
ATPase Trocadora de Sódio-Potássio
/
Hipertensão
Limite:
Animals
Idioma:
En
Ano de publicação:
1983
Tipo de documento:
Article