Novel liposome composition for a rapid colorimetric test for systemic lupus erythematosus.
Clin Chem
; 29(9): 1587-92, 1983 Sep.
Article
em En
| MEDLINE
| ID: mdl-6349851
ABSTRACT
This test for systemic lupus erythematosus utilizes a novel liposome composition entrapping the cation-responsive red dye Arsenazo III. In dilutions of normal sera the liposome membranes undergo a rearrangement when divalent cations are added, resulting in the release of the encapsulated dye and the rapid formation of a stable blue cation-dye complex. Microliter amounts of sera from patients with active lupus stabilize the liposome preparation such that the vesicles remain intact in the presence of the added divalent cations and thus maintain their red color for extended periods. The assay requires 1-min incubations in sera at room temperature and can be performed with standard microtiter plates, allowing the screening of large numbers of serum samples in a short time. Moreover, the unique absorption spectra of the complexed and uncomplexed dye allow for quantification of results.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arsenazo III
/
Compostos Azo
/
Lipossomos
/
Lúpus Eritematoso Sistêmico
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
1983
Tipo de documento:
Article