Polyclonal activation of canine B lymphocytes evaluated by a protein A reverse hemolytic plaque assay.
Vet Immunol Immunopathol
; 6(3-4): 353-64, 1984 Jul.
Article
em En
| MEDLINE
| ID: mdl-6385465
ABSTRACT
This paper describes the optimal culture and assay conditions for the polyclonal activation of canine lymphocytes with pokeweed mitogen and the quantitation of immunoglobulin secreting plaque-forming cells (PFC) using a staphylococcal protein A-reverse hemolytic plaque assay. The assay permits the quantitation of total immunoglobulin secreting PFC as well as class-specific immunoglobulin secreting PFC. On the optimal day of culture, a mean of 176 IgA PFC/10(6), 575 IgM PFC/10(6), 1276 IgG PFC/10(6), and 2158 total PFC/10(6) cells were generated following polyclonal activation. This study provides a simple and reproducible assay for the delineation of the immunoregulatory mechanisms involved in the differentiation of canine B lymphocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Ativação Linfocitária
/
Técnica de Placa Hemolítica
Limite:
Animals
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article