Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s).
J Exp Med
; 160(6): 1850-63, 1984 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-6439816
ABSTRACT
To gain insight into how T cell-derived lymphokines induce the secretion of IgG in activated B cells, we performed a limiting dilution analysis, using murine splenic B cells incubated with lipopolysaccharide (LPS) and a T cell-derived B cell differentiating factor for IgG (BCDF gamma)-containing supernatant (SN). The results of this analysis indicate that such a SN induces a marked increase in the precursor frequency of IgG1-secreting cells and a modest increase in clone size. The precursors lack surface IgG and are committed to the differentiation pathway for IgG1 secretion after LPS activation, but before the addition of BCDF gamma-containing SN. The majority of IgG1-secreting clones arise independently from precursors of cells that secrete IgG3. Taken together, these results indicate that BCDF gamma directs differentiation of activated B cells to IgG1 secretion.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina G
/
Linfócitos B
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Linfócitos T
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Linfocinas
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Células Clonais
Limite:
Animals
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article