Enrichment of MLR-activated human suppressor cells by discontinuous density-gradient centrifugation.
Immunobiology
; 158(5): 403-15, 1981 Jun.
Article
em En
| MEDLINE
| ID: mdl-6455379
ABSTRACT
6-day allogeneic mixed lymphocyte cultures (MLR) were fractionated on a four-step discontinuous Percoll gradient. The various fractions were added as regulatory cells to freshly established micro-MLRs between the original or third-party cell donors. Unspecific suppressor activity was highly enriched in gradient fraction III (density 1.069--1.062 g/ml), consisting mainly of blast-like cells. Suppressor cells were non-adherent T cells as determined by their spontaneous rosette formation with sheep erythrocytes and their susceptibility to lysis by anti-T-cell globulin plus complement. Mitomycin C treatment diminished their inhibitory capacity. In addition, gradient fraction III was also enriched with alloantigen-activated memory cells. By contrast, unprimed alloreactive cells were recovered from gradient fraction IV (density greater than or equal to 1.070 g/ml), consisting mainly of small, resting lymphocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ativação Linfocitária
/
Linfócitos
/
Linfócitos T Reguladores
Limite:
Adult
/
Humans
Idioma:
En
Ano de publicação:
1981
Tipo de documento:
Article