Blotting intact immunoglobulins and other high-molecular-weight proteins after composite agarose-polyacrylamide gel electrophoresis.
Anal Biochem
; 140(1): 208-13, 1984 Jul.
Article
em En
| MEDLINE
| ID: mdl-6486407
ABSTRACT
A composite agarose-polyacrylamide gel containing urea and sodium dodecyl sulfate reliably resolved unreduced human immunoglobulins according to their molecular weight. Intact immunoglobulins and a number of other macromolecules were readily transferred to nitrocellulose paper by either capillary or electrophoretic blotting, although the latter technique was more effective. Conventional antigen probing as well as immobilized antibody studies can be performed on the nitrocellulose transfers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulinas
/
Proteínas
Limite:
Humans
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article