Comparison of histologic and flow cytometric evaluation of cyclophosphamide-induced testicular damage.
Urology
; 24(5): 472-5, 1984 Nov.
Article
em En
| MEDLINE
| ID: mdl-6495459
This study evaluates the ability of DNA histograms obtained by flow cytometry to detect and quantify reversible alterations in spermatogenesis induced by cyclophosphamide, a known inhibitor of spermatogenesis. Evaluation of per cent of cells in each of the haploid (lc), diploid (2c), and tetraploid peaks (4c) as determined by flow cytometry in treated and control Balb/C mice over a six-week period, and comparison with routine histologic evaluation have led us to conclude that DNA histogram evaluation is a rapid and accurate means of identifying testicular damage and recovery. This technique may be useful in sequential monitoring of the effects of malignancy and/or treatments applied on spermatogenesis in young men.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatogênese
/
Testículo
/
Ciclofosfamida
/
Citometria de Fluxo
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article