Rapid and efficient purification of mouse monoclonal antibodies from ascites fluid using high performance liquid chromatography.
J Immunol Methods
; 69(1): 33-42, 1984 Apr 13.
Article
em En
| MEDLINE
| ID: mdl-6715888
ABSTRACT
Techniques for the rapid and efficient purification of mouse monoclonal antibodies from murine ascites are described that utilize anion exchange and gel permeation chromatography using high performance liquid chromatography (HPLC). Anion exchange chromatography was performed at neutral pH using a hydrophilic resin conjugated with a substituted amine (Mono Q column, Pharmacia Fine Chemicals). Various subclasses of mouse IgG monoclonals were assessed for their binding to this matrix, with all of the antibodies tested eluting at relatively low concentrations of sodium chloride. In some instances, a protein tentatively identified as transferrin was co-purified using this anion exchange procedure. However, this protein was easily removed from the IgG using gel permeation chromatography (Bio-Sil TSK-250, Bio-Rad), also performed at neutral pH.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Líquido Ascítico
/
Cromatografia Líquida de Alta Pressão
/
Anticorpos Monoclonais
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article