Determination of glycosylated haemoglobin by isoelectric focusing in non-linear pH gradients.
J Chromatogr
; 307(1): 103-10, 1984 Apr 13.
Article
em En
| MEDLINE
| ID: mdl-6725476
ABSTRACT
A new isoelectric focusing technique for the separation and quantitation of glycosylated haemoglobin (HbA1c) is described. By using an equimolar mixture of two separators (0.2 M beta-alanine + 0.2 M 6-aminocaproic acid) a 2-pH unit Ampholine range (pH 6-8) is transformed in a shallow, 0.6-pH unit span (pH 6.7-7.3). This brings about an increment of resolution between HbA and HbA1c by a factor of about three, thus allowing proper densitometric evaluation of the trichloroacetic acid-fixed MetHb bands by conventional gel scanners. Excellent agreement is found among microchromatography, isoelectric focusing followed by densitometry in situ, and isoelectric focusing followed by band excision, elution and spectrophotometric determination. The present method also allows full resolution between HbA1c and fetal haemoglobins (F and Fac bands).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hemoglobinas Glicadas
/
Focalização Isoelétrica
Limite:
Adult
/
Humans
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article