A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids.
Anal Biochem
; 138(1): 141-3, 1984 Apr.
Article
em En
| MEDLINE
| ID: mdl-6731838
ABSTRACT
A rapid method based on a defined methanol-chloroform-water mixture for the quantitative precipitation of soluble as well as hydrophobic proteins from dilute solutions (e.g., column chromatography effluents) has been developed. The effectiveness of this method is not affected by the presence of detergents, lipids, salt, buffers, and beta-mercaptoethanol.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas
Limite:
Animals
Idioma:
En
Ano de publicação:
1984
Tipo de documento:
Article