Venezuelan equine encephalomyelitis virus: concentration, partial purification, inactivation and immunogenicity.
Comp Immunol Microbiol Infect Dis
; 6(1): 31-7, 1983.
Article
em En
| MEDLINE
| ID: mdl-6825417
ABSTRACT
Venezuelan equine encephalomyelitis (VEE) TC-84 vaccinal virus, from 10-1. quantities of infected duck embryo fibroblast cell culture fluids, was isolated by combined continuous-flow centrifugation with isopycnic banding in sucrose. Most of the recovered infectivity and hemagglutinating activity were in a single band at a buoyant density (rho) of 1.2. About 90% of the total input protein (450-520 mg) was removed with the effluent, whereas most of the remaining 10% also banded at a rho of 1.2. Infectivity was inactivated with formalin at a final concentration of 0.05% at 37 degrees C for 24 hr. Formalin-inactivated virus retained its immunogenicity and induced VEE virus-specific antibody in horses and guinea pigs. The horses and those guinea pigs that received equivalent doses of vaccine survived after a challenge of their immunity with virulent VEE virus.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vacinas Virais
/
Vírus da Encefalite Equina Venezuelana
/
Encefalomielite Equina
/
Encefalomielite Equina Venezuelana
/
Anticorpos Antivirais
Limite:
Animals
País/Região como assunto:
America do sul
/
Venezuela
Idioma:
En
Ano de publicação:
1983
Tipo de documento:
Article