Solid-phase synthesis of thymosin beta 4: chemical and biological characterization of the synthetic peptide.
Biochemistry
; 22(4): 733-40, 1983 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-6838821
ABSTRACT
The chemical synthesis of thymosin beta 4 using a solid-phase procedure has been accomplished. The synthetic product was found to be homogeneous on paper electrophoresis at pH 6.5, high-performance liquid chromatography on a reversed-phase column, and isoelectric focusing using polyacrylamide gels. The synthetic material was also shown to be identical with the natural thymosin beta 4 by tryptic peptide mapping, amino acid compositional analyses, and polyacrylamide gel isoelectric focusing. Biologically, synthetic thymosin beta 4 was found to be as active as the natural compound in a terminal deoxynucleotidyltransferase induction assay and in a macrophage migration inhibition assay. The proposed structure of the peptide hormone was thus confirmed by a chemical synthesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timosina
/
Hormônios do Timo
Limite:
Animals
Idioma:
En
Ano de publicação:
1983
Tipo de documento:
Article