Enzymatic assay for cholesterol ester hydrolase activity.
J Lipid Res
; 19(7): 913-6, 1978 Sep.
Article
em En
| MEDLINE
| ID: mdl-712250
ABSTRACT
A rapid and accurate method is described for the assay of cholesterol ester hydrolase (CEH) activity. Aliquots of the enzyme-substrate incubation mixture are extracted into isopropanol. The free cholesterol concentration in each extract is determined enzymatically using a single aqueous reagent containing cholesterol oxidase and peroxidase. The free cholesterol remaining after the cholesterol ester hydrolase-catalyzed esterification is converted to delta 4-cholestenone and hydrogen peroxide (H2O2); peroxidase couples H2O2 with phenol and 4-amino-antipyrine to yield a stable rose-colored product absorbing at 500 nm. The method is highly reproducible and the values correlate well with those obtained with the chromatographic radioassay of CEH activity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hidrolases de Éster Carboxílico
/
Esterol Esterase
Idioma:
En
Ano de publicação:
1978
Tipo de documento:
Article