HLA antibodies cytotoxic only for B lymphocytes.
Tissue Antigens
; 10(4): 323-9, 1977 Oct.
Article
em En
| MEDLINE
| ID: mdl-72430
ABSTRACT
Of 28 unabsorbed sera from polytransfused individuals or multiparous women, which were not cytotoxic for total peripheral blood lymphocytes, 13 were found to react positively against B cell-enriched suspensions. These 13 sera were further characterized after platelet absorption-elution, as well as by pretreatment with turkey anti-human-beta2-microglobulin serum. Different patterns were found five sera behaved as pure anti-B cell reagents; four seemed to contain different antibody populations, directed against both B cell determinants and HLA-A, -B or -C antigens; four only contained antibodies directed against HLA-A, -B or -C specificities. Absorption experiments with purified T and B lymphocytes, showed that these last sera, although noncytotoxic for T cells, can be absorbed by them. It was concluded that not all sera reacting only with B lymphocytes recognize specifities absent from T cells, and that all sera should be exhaustively absorbed with platelets before being tested as anti-B cell-specific reagents.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Antígenos HLA
/
Isoanticorpos
/
Citotoxicidade Celular Dependente de Anticorpos
Limite:
Female
/
Humans
Idioma:
En
Ano de publicação:
1977
Tipo de documento:
Article