Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections.

Blattner, F R; Blechl, A E; Denniston-Thompson, K; Faber, H E; Richards, J E; Slightom, J L; Tucker, P W; Smithies, O

Blattner, F R; Blechl, A E; Denniston-Thompson, K; Faber, H E; Richards, J E; Slightom, J L; Tucker, P W; Smithies, O.

Science ; 202(4374): 1279-84, 1978 Dec 22.

Article em En | MEDLINE | ID: mdl-725603

ABSTRACT

ABSTRACT

Shotgun collections of Charon 3A bacteriophages containing Eco RI fragments of human and mouse DNA were constructed with the use of in vitro packaging. Plaques were screened by hybridization, and globin-specific clones were isolated from both human (Charon 3AHs51.1) and mouse (Charon 3AMm30.5). The fragments cloned were detected in unfractionated genomic DNA by the Southern method of hybridization.

Assuntos

Genes; Globinas/genética; Animais; Colífagos/genética; Enzimas de Restrição do DNA; DNA Recombinante; Hemoglobina Fetal/genética; Humanos; Métodos; Camundongos; Hibridização de Ácido Nucleico; Poli A; Poli T

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Globinas / Genes Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Animals / Humans Idioma: En Ano de publicação: 1978 Tipo de documento: Article

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