Gel chromatography of heparin.
J Chromatogr
; 226(1): 61-7, 1981 Nov 13.
Article
em En
| MEDLINE
| ID: mdl-7320156
ABSTRACT
It is assumed that heparin is a heterogeneous substance. In order to further investigate the purification of heparin, a column chromatographic technique for the fractionation of heparin is described using various diameters of bead form cross-linked dextran gels and an automated apparatus. It was observed that Sephadex G-50 resulted in the separation of three well formed peaks and provided superior resolution compared to all other gels. One of the peaks, representing 51% of the original material, possessed strong anticoagulant activity as measured by the recalcification time, partial thromboplastin time, thrombin time and the anti-Xa test. This peak also possessed strong metachromasia after electrophoresis as well as having a very potent anticoagulant effect in vivo. This technique may have a significant role in the purification of this agent from tissue sources.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Heparina
Limite:
Animals
Idioma:
En
Ano de publicação:
1981
Tipo de documento:
Article