Effects of LDL, HDL and their combination on the endogenous cholesterol synthesis in monocyte macrophages.
Biochim Biophys Acta
; 666(3): 405-10, 1981 Dec 23.
Article
em En
| MEDLINE
| ID: mdl-7326250
ABSTRACT
It is commonly assumed that the low density lipoprotein (LDL) degradation in extra-hepatic cells proceeds partly by way of the high-affinity LDL uptake process. In addition, it has been suggested that extra-hepatic LDL catabolism proceeds partly by way of preferential uptake and subsequent degradation of LDL by phagocytic scavenger cells. In order to study the effect of LDL and high density lipoprotein (HDL) on cholesterol metabolism in such scavenger cells, cultured human or pig monocytes were incubated with varying amounts of human or pig LDL, HDL and LDL/HDL mixtures, and the incorporation of [14C]acetate into cholesterol was measured. The addition of LDL suppressed endogenous cholesterol synthesis. Incubation of monocytes with LDL/HDL mixtures reduced the total amount of de novo synthesized cholesterol to the same extent as incubation with LDL alone. Our results suggest that in cultured monocyte macrophages HDL does not influence endogenous cholesterol synthesis, even in the presence of LDL.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Monócitos
/
Colesterol
/
Lipoproteínas HDL
/
Lipoproteínas LDL
/
Macrófagos
Limite:
Animals
/
Humans
/
Male
/
Newborn
Idioma:
En
Ano de publicação:
1981
Tipo de documento:
Article