Alanine influx across serosal border of Testudo graeca intestine.

ABSTRACT

The influx of alanine across the serosal membrane of Testudo graeca intestinal cells with preserved epithelial orientation was examined. Our results suggest that 1. The mechanism of alanine influx across the serosal membrane of turtle intesintal cells is a carrier-mediated process that has the characteristics of facilitated diffusion. 2. Alanine influx mechanism is independent of intra- and extra-cellular changes in Na+ and K+ concentrations, and is not altered by reversal of Na+ and K+ gradients across the serosal membrane. 3. In Na+-free media the mechanism of transport of alanine at the mucosal membrane has the same pattern of competitive inhibition by amino acids as the serosal.