Localization of a vitronectin binding region of plasminogen activator inhibitor-1.
Thromb Haemost
; 73(5): 829-34, 1995 May.
Article
em En
| MEDLINE
| ID: mdl-7482411
ABSTRACT
The PAI-1 binding site for VN was studied using two independent methods. PAI-1 was cleaved by Staph V8 protease, producing 8 fragments, only 2 of which bound to [125I]-VN. These fragments were predicted to overlap between residues 91-130. Since PAI-2 has structural homology to PAI-1, but does not bind to vitronectin, chimeras of PAI-1 and PAI-2 were constructed. Four chimeras, containing PAI-1 residues 1-70, 1-105, 1-114, and 1-167 were constructed and expressed in vitro. PAI-1, PAI-2, and all of the chimeras retained inhibitory activity for t-PA, but only the chimera containing PAI-1 residues 1-167 formed a complex with VN. Together, these results predict that the VN binding site of PAI-1 is between residues 115-130.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Estrutura Terciária de Proteína
/
Inibidor 1 de Ativador de Plasminogênio
/
Vitronectina
Limite:
Humans
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article