The jellyfish green fluorescent protein: a new tool for studying ion channel expression and function.
Neuron
; 14(2): 211-5, 1995 Feb.
Article
em En
| MEDLINE
| ID: mdl-7531985
ABSTRACT
Two methods are described for using the jellyfish green fluorescent protein (GFP) as a reporter gene for ion channel expression. GFP fluorescence can be used to identify the transfected cells, and to estimate the relative levels of ion channel expression, in cotransfection experiments. A GFP-NMDAR1 chimera can be constructed that produces a functional, fluorescent receptor subunit. These methods should facilitate studies of ion channel expression, localization, and processing.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptores de N-Metil-D-Aspartato
/
Canais Iônicos
/
Proteínas Luminescentes
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article