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Effectiveness of a dot-blot immunoassay of anti-Rickettsia tsutsugamushi antibodies for serologic analysis of scrub typhus.
Weddle, J R; Chan, T C; Thompson, K; Paxton, H; Kelly, D J; Dasch, G; Strickman, D.
Afiliação
  • Weddle JR; Viral and Rickettsial Diseases Program, Naval Medical Research Institute, Bethesda, Maryland, USA.
Am J Trop Med Hyg ; 53(1): 43-6, 1995 Jul.
Article em En | MEDLINE | ID: mdl-7625532
We compared a commercially available dot-blot immunoassay system with the indirect immunofluorescence assay (IFA) in tests of known negative and known positive sera from scrub typhus cases. Using a panel of 100 sera from patients with various rickettsial and nonrickettsial infections, we observed that the IFA was 99% specific and the dipstick assay was 98% specific. In tests of 91 sera (30 negative and 61 positive for scrub typhus antibodies) from a study of febrile patients in Malaysia, using the standard of an IFA titer < 1:64 as negative, an IFA titer > 1:128 as positive, and an IFA titer = 1:64 as either positive or negative (supported by clinical records), dipsticks were 83% specific and 90% sensitive. The quantitative correlation of the dipsticks to IFA titers was confirmed by significant differences in geometric means of inverse IFA titers corresponding to the number of positive dipstick spots (no dots = 8.5, one dot = 43.3, two dots = 206.7, and three dots = 676.9). The assay would enable physicians and public health workers who deal with patients to quickly diagnose and appropriately treat most cases of the disease, especially in areas of high prevalence where the proportion of false-positive results to true-positive results would be low.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Orientia tsutsugamushi / Tifo por Ácaros / Immunoblotting / Imunofluorescência / Anticorpos Antibacterianos Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Humans País/Região como assunto: Asia Idioma: En Ano de publicação: 1995 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Orientia tsutsugamushi / Tifo por Ácaros / Immunoblotting / Imunofluorescência / Anticorpos Antibacterianos Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Limite: Humans País/Região como assunto: Asia Idioma: En Ano de publicação: 1995 Tipo de documento: Article