Measurement of antibody and antigen concentrations with a sodium sulphate glutaraldehyde technique.
J Immunol Methods
; 20: 287-95, 1978.
Article
em En
| MEDLINE
| ID: mdl-77296
ABSTRACT
Gamma-globulins can be crosslinked at low concentrations by simultaneous treatment with sodium sulphate and glutaraldehyde (SSG-method). This precipitate can be used for the assay of antibody or antigens. Examples are given for the measurement of (1) anti-dinitrophenyl antibody by reaction of the precipitate with 125I-dinitrophenylovalbumin, (2) IgA by inhibition of an anti-IgA precipitate with an 125I-labelled IgA myeloma protein, (3) idiotypic antibody by inhibition of the binding of a 125I-labelled myeloma protein by an anti-idiotype precipitate.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sulfatos
/
Glutaral
/
Aldeídos
/
Anticorpos
/
Antígenos
Limite:
Animals
Idioma:
En
Ano de publicação:
1978
Tipo de documento:
Article