Genetic improvements of an industrial strain of Aspergillus flavus for urate oxidase production.
J Biotechnol
; 27(3): 239-47, 1993 Feb.
Article
em En
| MEDLINE
| ID: mdl-7763466
ABSTRACT
Urate oxidase, an enzyme used in human therapy, is currently produced industrially by a strain of Aspergillus flavus. Two strategies of strain improvement were tested in order to obtain higher yields of urate oxidase. The first one, based on a classical mutation-selection protocol, led to the isolation of a mutant strain that overproduced uricase two-fold as compared to the industrial strain. The second one consisted in the construction of transformed strains that had integrated multiple copies of a urate oxidase-expression vector. A twenty-fold improvement in urate oxidase was obtained by this method.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aspergillus flavus
/
Urato Oxidase
Tipo de estudo:
Guideline
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article