Production of a phosphorylated GST::HPV-6 E7 fusion protein using a yeast expression vector and glutathione S-transferase fusions.
Gene
; 152(1): 137-8, 1995 Jan 11.
Article
em En
| MEDLINE
| ID: mdl-7828922
ABSTRACT
A Saccharomyces cerevisiae GAL7 expression vector for the production of protein fusions to glutathione S-transferase (GST) has been constructed. Using this vector, a GST fusion to human papillomavirus type 6 (HPV-6) E7 protein was produced and purified by affinity chromatography in a single step, at a yield of 2 micrograms/ml of culture. The E7 portion of the fusion protein was phosphorylated, in contrast to the same product made in Escherichia coli. Therefore, yeast GST vectors may be of specific use in producing phosphoproteins, or proteins with other eukaryotic post-translational modifications, in preparative amounts for in vitro analysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
Fosfoproteínas
/
Saccharomyces cerevisiae
/
Proteínas Oncogênicas Virais
/
Vetores Genéticos
/
Glutationa Transferase
Idioma:
En
Ano de publicação:
1995
Tipo de documento:
Article