Endothelium-denuded pulmonary resistance arteries from the fetal lamb: preparation and response to vasoactive agents.
J Pharmacol Toxicol Methods
; 32(2): 85-91, 1994 Oct.
Article
em En
| MEDLINE
| ID: mdl-7865866
ABSTRACT
A method was developed for preparing endothelium-denuded resistance arteries (mean internal diameter, 176 microns) from the term fetal lung to be used for tests in vitro. The endothelium was removed mechanically by passing a sandpaper-polished cat whisker through the lumen. Ultrastructurally, the preparation had a well-preserved internal elastic lamina which was facing the lumen without any endothelium superimposed. Its response to contractile agents (excess potassium, a thromboxane A2 analogue, endothelin-1) tended to be greater compared to the intact artery. Conversely, the relaxation to acetylcholine was abolished in the endothelium-denuded artery and, in its place, a modest contraction ensued. Sodium nitroprusside relaxation, unlike acetylcholine relaxation, was affected insignificantly by endothelium denudation. This preparation, in combination with the preparation of the intact resistance artery, is a useful, new tool for studying local factors responsible for pulmonary hemodynamic control in utero and through the transitional period at birth.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasoconstritores
/
Vasodilatadores
/
Circulação Pulmonar
/
Pulmão
/
Músculo Liso Vascular
Limite:
Animals
Idioma:
En
Ano de publicação:
1994
Tipo de documento:
Article