A novel, specific pro-urokinase complex on monocyte-like cells, detected by transglutaminase-catalyzed cross-linking.
FEBS Lett
; 336(3): 394-6, 1993 Dec 28.
Article
em En
| MEDLINE
| ID: mdl-7904248
ABSTRACT
Radiolabeled pro-urokinase plasminogen activator (pro-uPA) was cross-linked to a specific protein on the surface of human monocyte-like U937 cells in a reaction catalyzed by tissue transglutaminase. The conjugate formed with this unknown component had a much higher molecular weight (apparent M(r) 250,000-300,000) than the complex of pro-uPA and the urokinase plasminogen activator receptor (uPAR). There was a strong preference for the pro-form of uPA. The conjugate was recognized by antibodies against uPA but not by anti-uPAR antibodies. Nevertheless, the blocking of uPAR with a monoclonal antibody abolished the formation of the conjugate, thus showing a role of uPAR in this process.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Monócitos
/
Ativador de Plasminogênio Tipo Uroquinase
/
Transglutaminases
/
Reagentes de Ligações Cruzadas
Limite:
Humans
Idioma:
En
Ano de publicação:
1993
Tipo de documento:
Article